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KMID : 0358619950290020225
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Korean Journal of Physiology
1995 Volume.29 No. 2 p.225 ~ p.232
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Phorbol Ester-Induced Periodic Contraction in Isolated Rabbit Jugular Vein
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Ryu Jae-Cheol
Jung Dong-Keun
Lee Sang-Ho
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Abstract
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The present study was conducted to evaluate the effect of phorbol 12,13-dibutyrate (PDBu) on the contraction of rabbit jugular vein in vitro. PDBu concentrations of greater than 10 nM induced a periodic contraction which was composed of rapid contraction, plateau and slow relaxation. The frequency of periodic contraction increased as PDBu concentration increased. The PDBu-induced contraction was inhibited by staurosporine (100 nM), it was not changed by tetrodotoxin In -free medium, PDBu induced a sustaining contraction, but not periodic contraction. Addition of to medium evoked periodic contraction which was inhibited by nifedipine, PDBu concentrations of greater than increased uptake without changing efflux. Charybdotoxin and apamin, -activated channel blockers, did not affect the PDBu-induced periodic contraction, whereas tetraethylammonium (TEA) abolished the periodicity. Pinacidil , a potassium channel activator, blocked PDBu induced periodic contraction, which was recovered by glybenclamide . In high potassium solution, PDBu did not produce the periodic contraction. These results suggest that the PDBu-induced periodicity of contraction is modulated by voltage dependent channel and ATP-sensitive channel.
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KEYWORD
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Phorbol ester, Periodic contraction, Jugular vein
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